lab-tools
Single-file browser tools for the bench. No build step, no server, nothing sent anywhere.
sgRNA oligo designerProtospacer in, annealed cloning oligos out. Two overhang sets, checks, batch paste and a printable order sheet. BCA loading calculatorA562 against a standard curve, then the western loading recipe: lysate, buffer, β-mercaptoethanol, water. Viral titer and MOITiter from percent-positive, then the transduction setup per well. Warns on saturated dilutions. Lentivirus packaging calculatorpsPAX2 + pCMV-VSV-G with TransIT-LT1 into 293T, scaled by vessel. LV900 titer calculatorFits Ct against ln(titer) across the SBI LV900 standards.