Coculture plate designer

Fill the blocks → the plate and the pipetting volumes update as you type.

Start from a design you already have

Open a plate_design.yaml that this page wrote earlier, or one you hand-wrote in the same format. The whole page fills in, painted wells included, and you edit it here instead of in a text editor. Nothing is written back to the file you open.

Experiment

Blocks down the plate (rows A–H)

Write the rows a condition covers: A-D, A, or A,C,E. Add a second layer when two things vary down the plate.

Blocks across the plate (columns 1–12)

Write the columns a condition covers: 1-3, 5, or 1,4,7.

Naming & odd wells

Seeding & pipetting

T cells — E:T is effector : target, effector first
Drug / BiTE dilutions

Plate

Paint wells by hand — these win over the blocks above

Pick a brush, then click or drag across the plate. Click a row letter or a column number to do the whole row or column. Painted wells beat the blocks, and they are written into override: in the design file.

Bench worklist

Get the files

This page cannot write into a folder on your computer, so it hands the design over as downloads.

The design .yaml carries the whole layout, the painted wells and the prep numbers, so it is the file to keep with the experiment, and it is what you open again at the top of this page. A companion script can turn that same .yaml into a printable plate map, an Excel layout and a worklist file; that script is not included with this page, so the .yaml and the .csv here are the two files you get.

Show the design file